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Journal of Botany
Volume 2014 (2014), Article ID 623651, 11 pages
Research Article

Phenolic Composition and Antioxidant and Antimicrobial Activities of Extracts Obtained from Crataegus azarolus L. var. aronia (Willd.) Batt. Ovaries Calli

1UR Morphogenesis and Plant Biotechnology Research Unit (UR/09-11), Faculty of Science Tunis, Campus Universitaire, 1060 Tunis El Manar, Tunisia
2Metabolic Biophysics and Applied Pharmacology Laboratory, Department of Biophysics, Faculty of Medicine of Sousse, University of Sousse, 4000 Sousse, Tunisia
3Laboratory of Plant Pathology, Higher Institute of Agronomy of Chott Mariem, 4042 Sousse, Tunisia
4Laboratory of Environment Microbiology, Faculty of Pharmacy, 5000 Monastir, Tunisia
5“Abiotic Stress and Cultivated Plants Differentiation” UMR1281 USTL, INRA, IFR147, Lille 1 University of Sciences and Technology, SN2 Building, 59655 Villeneuve d’Ascq Cedex, France
6Pharmacognosy Laboratory, Faculty of Pharmacy, BP 83, 59006 Lille, France
7Laboratory of Genetics Biodiversity and Valorisation of Bioressources (LR11ES41), Higher Institute of Biotechnology, Rue Tahar Haddad, 5000 Monastir, Tunisia

Received 21 August 2013; Accepted 9 December 2013; Published 20 January 2014

Academic Editor: Muhammad Y. Ashraf

Copyright © 2014 Radhia Bahri-Sahloul et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. Plant cell culture is an innovative technology to produce a variety of substances. Numerous plants synthesize among their secondary metabolites phenolic compounds which possess antioxidant and antimicrobial effects. Hawthorn (Crataegus) is one of these plants which has long been used in folk medicine and is widely utilized in pharmaceutical preparations mainly in neuro- and cardiosedative actions. Methods and Results. The production of polyphenol by fifty-two-week-old Crataegus azarolus var. aronia calli was studied in relation to growth variation and antioxidant and antimicrobial capacity within a subcultured period. The DPPH and ABTS+ assays were used to characterize the antioxidant actions of the callus cultures. Antimicrobial activity was tested by using disc diffusion and dilution assays for the determination of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) values of each active extract. High TEACDPPH, TEACABTS, and antimicrobial activity was observed when maximal growth was reached. An optimum of total phenol, proanthocyanidins, flavonoid, (−)-epicatechin, procyanidin B2, chlorogenic acid, and hyperoside was produced during this period. Conclusion. Antioxidant and antimicrobial activities were strongly correlated with total phenols and total flavonoids. Crataegus azarolus var. aronia cells culture represents an important alternative source of natural antioxidants and antimicrobials.