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Journal of Botany
Volume 2014 (2014), Article ID 647469, 6 pages
Research Article

A Novel Method to Overcome Coat-Imposed Seed Dormancy in Lupinus albus L. and Trifolium pratense L.

1Department of Agricultural Biotechnology, Faculty of Agriculture, Canakkale Onsekiz Mart University, Terzioglu Campus, 17020 Canakkale, Turkey
2Kahramanmaras Anadolu Oğretmen Lisesi, 46100 Kahramanmaras, Turkey

Received 7 May 2014; Revised 18 July 2014; Accepted 19 July 2014; Published 3 August 2014

Academic Editor: Bhagirath S. Chauhan

Copyright © 2014 Iskender Tiryaki and Mustafa Topu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We have developed a novel method to overcome coat-imposed seed dormancy in legume plants. Seeds of Lupinus albus L. and Trifolium pratense L. were stored in a freezer at −80°C for a period of time and then immediately treated with or without hot water at 90°C for 5 seconds. Germination tests were carried out in darkness at °C with four replications in a completely randomized design. Final germination percentage (FGP), germination rate, and synchrony of seeds were evaluated. The results showed that new approach of freeze-thaw scarification provided high percentage of germinations in white lupin (84.16%) and red clover (74.50%) seeds while control seeds had FGPs of 3.3% and 26.0%, respectively. The immediate thawing of frozen seeds in hot water for 5 seconds was found not only an effective and reliable but also the quickest seed treatment method to prevail against coat-imposed seed dormancy in legume species and may become operationally applicable to other plant species.