Research Article

Production and Metabolism of Indole Acetic Acid in Root Nodules and Symbiont (Rhizobium undicola) Isolated from Root Nodule of Aquatic Medicinal Legume Neptunia oleracea Lour.

Table 3

IAA production by different isolates obtained from the nodules of N. oleracea. Results presented are the mean of 3 individual experimental setups.

Isolated strainHost plantGrowth OD
at 540 nm
Production of IAA (μg/mL)

Rhizobium undicola strain N30Neptunia oleracea Lour.
Rhizobium undicola strain N32Neptunia oleracea Lour.
Rhizobium undicola strain N34Neptunia oleracea Lour.
Rhizobium undicola strain N35Neptunia oleracea Lour.
Rhizobium undicola strain N37Neptunia oleracea Lour.

The bacteria were grown in L-tryptophan (0.1%) supplemented yeast extract medium for 24 h at 30 ± 2°C. The control set was devoid of any carbon sources.