Production and Metabolism of Indole Acetic Acid in Root Nodules and Symbiont (Rhizobium undicola) Isolated from Root Nodule of Aquatic Medicinal Legume Neptunia oleracea Lour.

Table 4

Effect of different carbon sources on growth and IAA production by the Rhizobium undicola isolated from nodule of Neptunia oleracea (Lour.). The pairwise comparison showed significant differences under different carbon sources, growth, and indole acetic acid production of both the strains except those presented with NS (nonsignificant) in superscript. Here fructose and maltose for growth are nonsignificant (NS).

Carbon sources (0.2%)	Growth at 540 nm	IAA production (μg/mL)
Control
Mannose
Lactose
Fructose^NS
Sucrose
Maltose^NS
Galactose
Glucose
Mannitol

The bacteria were grown in L-tryptophan (0.2%) supplemented yeast extract medium for 24 h at 30 ± 2°C. The control set was devoid of any carbon sources. In others carbon sources were supplemented at 0.2% level individually. Results presented are the mean of 3 individual experimental setups.