Research Article
Inhibitory Effect of Heracleum persicum and Ziziphus jujuba on Activity of Alpha-Amylase
Table 1
Measuring of alpha-amylase activity and protein at different stages of purification.
| | Purification step | Total protein (mg) | Total activity (units) | Specific activity (U/mg) | Activity recovery (%) | Purification factor |
| | Crude extract | 10820 | 11600 | 1.07 | 100 | 1 | | (NH4)2SO4 precipitation (50–80%) | 4130 | 9810 | 2.37 | 84.5 | 2.21 | | Sephadex G-75 chromatography | 3.1 | 149.6 | 48.2 | 1.28 | 45 |
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