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Journal of Botany
Volume 2016 (2016), Article ID 7910451, 7 pages
Research Article

Preliminary Comparative Analysis of Phenological Varieties of Quercus robur by ISSR-Markers

1Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don 344 041, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117 997, Russia

Received 29 October 2015; Revised 16 January 2016; Accepted 21 January 2016

Academic Editor: Lorenzo Peruzzi

Copyright © 2016 Vasiliy Chokheli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Quercus robur L. is a valuable wood species having long ontogeny and promising to create long-living artificial plantings of recreational and ameliorative purposes in the steppes zone of Russia and other countries. In this work we have performed the genotyping of varieties of Quercus robur L. obtained from collection of Botanical Garden of Southern Federal University using intersimple sequence repeat (ISSR) molecular markers. The most polymorphic ISSR-marker (GA) 8YC was found in the collection. The polymorphic DNA markers identified in the present study can be used for the future breeding works to obtain valuable genotypes of Quercus genus. In addition we have performed DNA fingerprinting of the prospective sample of the variety Q. robur var. tardiflora Czern.