Journal of Botany

Journal of Botany / 2017 / Article / Tab 1

Research Article

Demucilaging Freshly Stored Seeds of Cocoa (Theobroma cacao L.) Improves Seedling Emergence and Growth

Table 1

(a) Two-way ANOVA of effects of seed storage duration and seed mucilage manipulation on plant height and number of leaves of cocoa on seedlings grown in soil-filled nursery bags for 12 weeks. Seed storage durations for intact and demucilaged seeds were up to 12 and 27 days, respectively. Data was not taken at weeks at 10 and 12 weeks for both demucilaged and intact mucilage seeds (data are means and ). (b) Two-way ANOVA of effects of seed storage duration and seed mucilage manipulation on leaf area and plant girth of cocoa on seedlings grown in soil-filled nursery bags for 12 weeks. Seed storage duration for intact and demucilaged seeds were up to 12 and 27 days, respectively. Data was not taken at weeks at 10 and 12 weeks for both demucilaged and intact mucilage seeds (data are means and ).
(a)

Measured traitSampling weekMucilage
status
Extracted seeds storage duration before sowing (d = days) valuel.s.d. (5%)
0 d3 d6 d9 d12 d15 d18 d21 d24 d27 d

Plant
height (cm)
Week 2Intact15.048.272.913.533.99<0.0012.457
Demucilaged1515.0215.0814.6514.7714.6915.1314.4813.35.67
Week 4Intact16.9610.043.46.855.57<0.0012.905
Demucilaged17.1717.0116.9817.021716.617.4416.4314.996.53
Week 6Intact17.759.413.897.85.63<0.0013.167
Demucilaged17.9118.0717.7717.3317.2319.3818.7517.7216.097.32
Week 8Intact19.149.432.535.615.6<0.0013.096
Demucilaged19.1719.1918.7318.3318.9120.9620.6719.1617.157.9
Week 10Intact21.999.960.795.484.77<0.0013.082
Demucilaged22.482221.6620.2721.02
Week 12Intact24.4110.110.734.753.99<0.0013.37
Demucilaged25.1324.9324.2423.924.91

Number of leavesWeek 2Intact4.32.70.90.91.1<0.0010.779
Demucilaged4.54.34.34.24.23.95.24.33.71.3
Week 4Intact5.93.71.42.41.6<0.0011.249
Demucilaged6.26.36.36.06.07.97.86.54.42.2
Week 6Intact9.33.91.72.91.9<0.0011.53
Demucilaged9.89.29.29.39.58.27.96.84.72.3
Week 8Intact11.02.70.42.72.5<0.0011.61
Demucilaged11.711.010.79.311.1109.710.36.64.4
Week 10Intact17.13.10.12.12.5<0.0012.585
Demucilaged17.317.117.017.116.0
Week 12Intact19.92.90.30.81.9<0.0012.159
Demucilaged20.120.119.519.019.9

(b)

Measured traitSampling weekMucilage
status
Extracted seeds storage duration before sowing (d = days)P valuel.s.d. (5%)
0 d3 d6 d9 d12 d15 d18 d21 d25 d27 d

Leaf area (cm2)Week 2Intact50.3719.175.995.362.96<0.0017.236
Demucilaged51.8148.5549.9244.7542.3151.548.543.638.916.9
Week 4Intact56.823.38.510.210.2<0.0019.82
Demucilaged56.158.654.752.553.599.273.864.648.326.5
Week 6Intact61.421.49.410.110<0.00112.53
Demucilaged67.260.76459.664.797.893.483.143.718.3
Week 8 Intact75.416.71.47.89.4<0.00116.77
Demucilaged80.377.473.971.574.5122.370113.771.334.7
Week 10Intact106.623.51.89.29.8<0.00118.73
Demucilaged112.8107.7109.4105.3106.6
Week 12Intact126.912.70.91.710.2<0.00120.72
Demucilaged129.5123.7122.2121126.1

Plant girth (mm)Week 2Intact3.32.10.81.40.8<0.0010.6576
Demucilaged3.33.33.33.33.33.23.23.02.81.2
Week 4Intact4.12.50.91.51.2<0.0010.7247
Demucilaged4.24.34.03.73.94.14.13.63.11.7
Week 6Intact4.92.80.91.01.6<0.0010.871
Demucilaged4.94.94.64.64.44.54.84.13.51.8
Week 8Intact5.12.71.01.51.5<0.0010.916
Demucilaged5.55.55.04.75.05.95.75.23.92.0
Week 10Intact6.12.90.61.50.8<0.0010.929
Demucilaged6.26.16.05.76.0
Week 12Intact6.82.70.31.71.2<0.0011.031
Demucilaged7.17.16.56.27.0

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