Antidepressant Interactions with the NMDA NR1-1b Subunit

Figure 4

ANS binding. To 5.29𝜇M NR1-1b S1S2 in buffer 3 was added ANS in a 1 : 1 molar ratio along with a TCA, SSRI, or SNRI in the following final concentrations: (a) TCAs-desipramine (- - -): 137𝜇M, trimipramine (⋯): 137𝜇M, maprotiline (■): 3.18 mM; (b) TCAs-nortriptyline (⋯): 2.08 mM; (c) SNRI-venlafaxine (- - -): 2.76 mM; (d) SSRI-fluvoxamine (- - -): 2.81 mM, paroxetine (⋯): 3.75 mM, sertraline (■): 1.88 mM; (e) SSRI-fluoxetine (- - -): 862𝜇M. In all figures the solid line is the extrinsic fluorescence of ANS:NR1-1b S1S2. In all figures, intensities were corrected by subtracting the drug and ANS blank.

(a)

(b)

(c)

(d)

(e)