Table of Contents
Journal of Biophysics
Volume 2008 (2008), Article ID 602639, 13 pages
Research Article

Selective Detection of NADPH Oxidase in Polymorphonuclear Cells by Means of NAD(P)H-Based Fluorescence Lifetime Imaging

1Institute of Physical and Theoretical Chemistry, University Braunschweig, Hans-Sommer Stra├če 10, D-38106 Braunschweig, Germany
2Helmholtz Centre for Infection Research, Inhoffenstra├če 7, D-38124 Braunschweig, Germany
3Centre for Immunology and Infection, Department of Biology, University of York, P.O. Box 373, York YO10 5YW, UK
4LaVision Biotec GmbH, Meisenstr. 65, D-33607 Bielefeld, Germany
5Institute of Molecular and Clinical Immunology, Otto von Guericke University Magdeburg, Leipzigerstrasse 44, D-39120 Magdeburg, Germany

Received 28 May 2008; Accepted 2 September 2008

Academic Editor: Peter T. C. So

Copyright © 2008 R. Niesner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Movie 1: CTO stained PMNs (red) interacting with FITC stained conidia (green, small, round) and hyphae (green, ribbon-shaped). The movie is a time series of 3D fluorescence images recorded every 60 seconds Note that the PMNs interact with hyphae both along their length (center) as well as with their conidiophores (right corner), that is, the part of hypha responsible for the asexual reproduction.

Movie 2: The movie is a time series of fluorescence lifetime images of enzyme-bound NAD(P)H of PMNs interacting with an aspergillus hypha recorded every 60 seconds Note that only the PMNs, which contact the hypha shows an increased fluorescence lifetime specific for the NADPH bound to NADPH oxidase. Moreover, this specific fluorescence lifetime appears only during the contact between cell and fungus, meaning that it appears and disappears a few times within the measuring period (30 minutes).

  1. Movie 1
  2. Movie 2