Crystal Structural and Functional Analysis of the Putative Dipeptidase from Pyrococcus horikoshii OT3

Table 6

Comparison of hydrophobic interaction due to dimer formation of A and B subunits for Ph dpd, Zn-Ph dpd, and Zn-Pfprol.

	Phdpd	Zn-Phdpd	Zn-Pfprol	Δ( = Ph dpd-Zn-Phdpd)	Δ( = Ph dpd-Zn-Pfprol)
ΔASAnp=(monomer^∗−dimer)	1396	2341	2263	−945	−867
ΔASAp=(monomer^∗−dimer)	914	1067	1070	−153	−156
Total buried area (Å^2)	2310	3408	3333
Δ𝐺HP (kJ/mol)	191	333	320	−142	−129

∗Monomer means the summation of ASA of A- and B-subunits.
ASA_np and ASA_p represent accessible surface area of nonpolar (C and S) atoms and polar (N and O) atoms, respectively.
The ASA values were calculated using software [20]. The unit of the ASA value is Ų . Δ𝐺_HP was calculated using (1).