Research Article

Exploring the Membrane Mechanism of the Bioactive Peptaibol Ampullosporin A Using Lipid Monolayers and Supported Biomimetic Membranes

Figure 2

Interaction of AmpA with eggPC monolayers at the air/water interface. (a) Kinetic (area versus time) analysis of AmpA insertion in eggPC monolayers at the air/water interface for different peptide subphase concentrations at 𝑃 = 3 0  mN·m−1. Arrows indicate AmpA injection times. (b) Brewster angle microscopy images at the air/water interface recorded after injection of AmpA at increasing concentrations. GL: gray levels obtained with a shutter speed of 50 s−1. Arrows on photographs point to the mixed lipid-peptide aggregates. (c) Collapse pressure values were measured upon compression of the film formed at the air/water interface by monitoring pressure-area isotherms.
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