pH-Dependent Interaction between C-Peptide and Phospholipid Bicelles

Figure 2

(a) 2D TOCSY spectra recorded at 600 MHz for 200 𝜇M C-peptide in 50 mM citrate buffer pH 3.2 (red) and in 50 mM sodium phosphate buffer pH 5.8 (black). (b) Differences in amide proton chemical shifts between pH 3.2 and pH 5.8 (i.e., amide proton shift pH 5.8 and amide proton shift pH 3.2) are plotted against differences in α proton chemical shifts (i.e., 𝛼 proton shift pH 5.8 and 𝛼 proton shift pH 3.2).