Thermal Aggregation of Recombinant Protective Antigen: Aggregate Morphology and Growth Rate

Table 2

Changes in enthalpy (Δ𝐻), entropy (Δ𝑆), Gibbs free energy (Δ𝐺) at 25°C, and denaturation temperature (𝑇_𝑚) for rPA unfolding (𝑁↔𝐼) with and without 0.25 M urea.

	Δ𝐻/kJ mol^−1	Δ𝑆/kJ mol^−1 K^−1	Δ𝐺 at 25°C/kJ mol^−1	𝑇𝑚 (Δ𝐺=0)/°C
rPA without additive	975.8 ± 40.5	3.02 ± 0.13	76.1	50.2
rPA with 0.25 M urea	949.7 ± 94.6	2.92 ± 0.30	77.7	51.6