Table of Contents
Journal of Cell Adhesion
Volume 2014 (2014), Article ID 860427, 6 pages
http://dx.doi.org/10.1155/2014/860427
Research Article

A Novel Utility for an Old Method: The Leukocyte Adherence Inhibition Test Is an Easy Way to Detect the Immunoreactive Interference of the Collection Tube Anticoagulant on Cellular Immunoassays

1Instituto Alergoimuno de Americana, Rua Chile, 689 Bairro Cechino, 13465-740 Americana, São Paulo, Brazil
2Faculty of Nursing, Anhanguera University of Santa Bárbara, São Paulo, Brazil
3Faculty of Biomedicine, Anhanguera University of Santa Bárbara, São Paulo, Brazil
4Faculty of Pharmacy, Methodist University of Piracicaba, São Paulo, Brazil

Received 7 December 2013; Revised 24 December 2013; Accepted 9 January 2014; Published 18 February 2014

Academic Editor: Seetharama D. Satyanarayanajois

Copyright © 2014 Celso Eduardo Olivier et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The leukocyte adherence inhibition (LAI) test was designed to evaluate the presence of cellular immunoreactivity against specific antigens and is highly sensitive to analytical interference. To compare the performance of the LAI test among plasmas obtained with different anticoagulants, 36 blood samples of allergic subjects were divided into three tubes with heparin, sodium citrate, and EDTA. The adherence was assessed with the hemocytometer technique. The mean adherence of each group was compared as well as the number of tests with inappropriate adherence (<25%). Of the 36 tests, there were 4, 9, and 21 with inappropriate adherence in the groups of heparin, citrate, and EDTA, respectively. Of these last, 5 samples also did not demonstrate proper adherence with sodium citrate and 1 sample did not show proper adherence with any anticoagulant. The anticoagulant in the collection tube can elicit a specific immune response, becoming an interferent for the LAI test. In this case, there is the possibility of redoing the test using another anticoagulant. Performed with different anticoagulants, the LAI test may be used as a preanalytical tool for choosing the less immunoreactive anticoagulant for more complex immunoassays.