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E-Journal of Chemistry
Volume 4, Issue 4, Pages 536-545
http://dx.doi.org/10.1155/2007/480907

Analysis of Three Penicillin Antibiotics (Ampicillin, Amoxicillin and Cloxacillin) of Several Iranian Pharmaceutical Companies by HPLC

A. Ashnagar1 and N. Gharib Naseri2

1School of Pharmacy, Ahwaz Jundi Shapour Univ. of Medical Sciences, Ahwaz, Iran
2Ahwaz Faculty of Petroleum Engineering, Petroleum Univ. of Technology, Iran

Received 4 March 2007; Accepted 30 March 2007

Copyright © 2007 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Penicillin has been the most widely used antibiotic for many gram-positive bacterial infections. In this research the purity of the standard active ingredients of the various dosage forms of three penicillins (Amoxicillin, Cloxacillin and Ampicillin) imported and the purity percentage of the active ingredients in each of the various dosage forms of these drugs manufactured by several pharmaceutical companies of Iran (Kosar, Farabi and Jaber Ibn Hayan) were investigated and determined by HPLC technique. The analyses were made by using a Knauer (Germany) Spherimage-80, ODS, 2-5 μm C18 column with 30 cm length, and i.d. 4.5 mm. A 20 μL solution from each individual sample and the standard solution were injected separately onto the column of an HPLC instrument which was equipped with ECW 2000 software of Knauer, Germany. The results obtained in this research have shown that the purity percentage of the active ingredients of the standard powder and the various dosage forms of all the drugs used, were 100%.