Journal of Chemistry

Journal of Chemistry / 2007 / Article

Open Access

Volume 4 |Article ID 632904 | 9 pages | https://doi.org/10.1155/2007/632904

Synthesis of Molecularly Imprinted Polymerand its Molecular Recognition Propertiesof N-Acetylneuraminic Acid

Received11 Apr 2007
Accepted20 Jul 2007

Abstract

A molecular imprinting technique was applied in this work to detect N-acetylneuraminic acid (Neu5Ac) and its analogue structure. Twomolecularly imprinted polymers (MIP) were prepared using Neu 5Ac as the template molecule, as well as methacrylic acid (MAA) or 4-vinylpyridine (4-Vpy) and ethyleneglycol dimethacrylate (EGDMA) as the functionalmonomer and cross-linker, respectively. Free radical polymerization was carried out at 4°C under UV radiation or thermal (60°C) polymerization. MIP thus obtained were ground into 11∼25µm and 25-44µm. The binding results from Neu5Ac solution, mannose (Man) solution and N-acetyl-Dmannosamine (ManNAc) solution performed by Neu5Ac-MIP showed specific binding toward Neu5Ac rather than other analogue compounds on the host-guest system. The values of capacity for Neu5Ac-MIP were measured and experimental results were further used for simulation to obtain the binding isotherms. The principal advantage of this method is thatNeu5Ac- MIP can recognize Neu5Ac and its analogue compounds.

Copyright © 2007 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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