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E-Journal of Chemistry
Volume 4, Issue 3, Pages 440-449

in vitro Antioxidant Activity and Total Polyphenolic Content of Cyperus rotundus Rhizomes

KR. Nagulendran, S. Velavan, R. Mahesh, and V. Hazeena Begum

Department of Siddha Medicine, Faculty of Science, Tamil University, Vakaiyur, Thanjavur-613 005, Tamilnadu, India

Received 28 February 2007; Accepted 30 March 2007

Copyright © 2007 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this study, Antioxidant activity of Cyperus rotundus rhizomes extract (CRRE) was evaluveted in a series of in vitro assay involving free radicals and reactive oxygen species and IC50 values were determined. CRRE exhibited its scavenging effect in concentration dependent manner on superoxide anion radicals, hydroxyl radicals, nitric oxide radical, hydrogen peroxide, and property of metal chelating and reducing power. The extract was also studied for lipid peroxidation assay by thiobarbituric acid–reactive substances (TBARS) using young and aged rat brain mitochondria. The extract was also effective in preventing mitochondrial lipid peroxidation induced by FeSO4/ ascorbate in concentration dependent manner. The results obtained in the present study indicate that C. rotundus rhizomes extract can be a potential source of natural antioxidant.