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E-Journal of Chemistry
Volume 5 (2008), Issue 3, Pages 427-430

High Performance Thin Layer Chromatographic Method for Estimation of Cefprozil in Tablet Dosage Form

V. Jagapathi Raju and J. V. L. N. Seshagiri Rao

University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530003, India

Received 19 May 2007; Accepted 10 July 2007

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple, fast, specific and precise HPTLC method has been developed for the estimation of cefprozil in pure and in tablet dosage forms. Aluminium plates precoated with silica gel G60F254 were used as stationary phase and a mixture of chloroform: methanol: toluene: diethyl amine: water in the ratio 4: 4.4: 3.2: 3: 0.8 v/v as mobile phase. Quantification was carried out by the use of a densitometer in absorbance mode at 286 nm. The Rf value of cefprozil was found to be between 0.37 and 0.40. The results of the analysis have been validated statistically and by recovery studies. Linearity was observed in the concentration range of 400-2000 ng/spot.