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E-Journal of Chemistry
Volume 5, S2, Pages 1114-1116

Spectrophotometric Estimation of Amoxicillin Trihydrate in Bulk and Pharmaceutical Dosage Form

K. Prakash,1 P. Narayana Raju,2 K. Shanta Kumari,1 and M. Lakshmi Narasu3

1SN Vanitha Mahavidyalaya Pharmacy College for Women, Nampally, Hyderabad, Andhra Pradesh, India
2St Peter's Institute of Pharmaceutical sciences, Hanmakond, Warangal. Andhra Pradesh, India
3Centre for Biotechnology, Institute of Science and Technology, JNTU, Kukatpally, Hyderabad, India

Received 12 December 2007; Accepted 20 January 2008

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


New simple, sensitive, rapid, reproducible and economical spectrophotometric method have been developed for the determination of amoxicillin trihydrate in pharmaceutical bulk and tablet dosage form using citro phosphate buffer pH 7.2. The system obeys Lambert-Beer's law at 231 nm in the concentration range 2.5-50 μg/m. Molar absorptivity, correlation coefficient and Sandell's sensitivity values were found to be 1.0020 x 104 mol-1 cm, -1 0.9996 and 0.03906 μg cm-2 respectively. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate