Journal of Chemistry

Journal of Chemistry / 2008 / Article

Open Access

Volume 5 |Article ID 385972 |

A. Lakshmana Rao, B. N. V. Ravi Kumar, G. G. Sankar, "Development of RP-HPLC Method for the Estimation of Rabeprazole in Pure and Tablet Dosage Form", Journal of Chemistry, vol. 5, Article ID 385972, 5 pages, 2008.

Development of RP-HPLC Method for the Estimation of Rabeprazole in Pure and Tablet Dosage Form

Received18 Mar 2008
Accepted10 May 2008


A simple, rapid, sensitive and precise High Performance Liquid Chromatographic (HPLC) method has been developed for the estimation of rabeprazole in bulk and tablet dosage form. In this method RP-C18 column (150 mm x 4.6 mm I.D, 5 µ m particle size) with mobile phase consisting of methanol and water in the ratio of 65:35 v/v in isocratic mode was used. The detection wavelength is 284 nm and the flow rate is 0.8 mL/min. Tinidazole is used as internal standard. In the range of 0.25-20 µ g/mL, the linearity of rabeprazole shows a correlation coefficient of 0.9999. The drug and internal standard were eluted at 4.41± 0.05 and 2.16± 0.04 min. respectively. The intra- and inter-day variation was found to be less than 1% showing high precision of the assay method. The detection limit was found to be 100 ng/mL. The mobile phase selected for the proposed method is simple, fast, accurate and precise and hence can be applied for routine quality control analysis of rabeprazole in bluk and its tablet dosage from.

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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