Journal of Chemistry

Journal of Chemistry / 2008 / Article

Open Access

Volume 5 |Article ID 425974 |

Shikha M. N. Roy, Kiran V. Mangaonkar, Santosh. M. Yetal, Santosh. S. Joshi, "LC-MS-MS Method for Determination of Metolazone in Human Plasma", Journal of Chemistry, vol. 5, Article ID 425974, 7 pages, 2008.

LC-MS-MS Method for Determination of Metolazone in Human Plasma

Received07 Aug 2007
Revised30 Oct 2007
Accepted20 Dec 2007


A rapid, sensitive and specific method for quantification of metolazone in human plasma using metaxalone as internal standard is described. Sample preparation involved a simple liquid-liquid extraction procedure. The extract was analyzed by high performance liquid chromatography coupled to electrospray tandem mass spectrometry (LC–MS–MS). Chromatography was performed isocratically on a 5 μm C18 analytical column (50 mm × 4.6 mm i.d.) with buffer–acetonitrile 20:80 (v/v) as mobile phase. The response to metolazone was a linear function of concentration over the range 1.00 to 2000.00 ng mL-1. The lower limit of quantification in plasma was 1.0 ng mL-1. The method was successfully applied in a bioequivalence study of a metolazone formulation after administration as a single oral dose.

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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