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E-Journal of Chemistry
Volume 6, Issue 2, Pages 469-474
http://dx.doi.org/10.1155/2009/157868

Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation

Zaheer Ahmed, B. Gopinath, A. Sathish Kumar Shetty, and B. K. Sridhar

Department of Pharmaceutical Analysis, National College of Pharmacy, Shimoga-577201, (K.S.), India

Received 15 September 2008; Accepted 1 November 2008

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A rapid and sensitive RP-HPLC method with UV detection (262 nm) for routine analysis of adefovir dipivoxil in bulk and in pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of acetonitrile and phosphate buffer (50:50, v/v) with flow rate 1.0 mL min-l. In the range of 5.0-100 µg/mL, the linearity of adefovir dipivoxil shows a correlation co-efficient of 0.9999. The proposed method was validated by determining sensitivity accuracy, precision, robustness stability, specificity, selectivity and system suitability parameters.