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E-Journal of Chemistry
Volume 6, Issue 2, Pages 332-340
http://dx.doi.org/10.1155/2009/194653

Analytical Study for the Charge-Transfer Complexes of Pregabalin

Hesham Salem

Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt

Received 3 September 2008; Accepted 1 November 2008

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Studies were carried out, for the first time, to investigate the charge-transfer reactions of Pregabalin (PRE) as n-electron donor with various π-acceptors: 7,7,8,8-tetracyanoquinodimethane (TCNQ), 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), 2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone (chloranilic acid, pCA), tetracyanoethylene (TCNE) and 2,3,5,6-tetrachloro-1,4-benzoquinone (chloranil). Different colored charge-transfer complexes and radical anions were obtained. Different variables affecting the reactions were studied and optimized. The formations of the colored complexes were utilized in the development of simple, rapid and accurate spectrophotometric methods for the analysis of PRE in pure form as well as in its pharmaceutical preparation. Under the optimum reaction conditions, linear relationships with good correlation coefficients (0.9995-0.9999) were found between the absorbance and the concentrations of PRE in the range of 8-400 µg mL-1. The limits of assays detection ranged from 0.60 to 8.11 µg mL-1. No interference could be observed from the additives commonly present in the capsules. The methods were successfully applied to the analysis of capsules that contain PRE, with good accuracy and precision; the recovery percentages ranged from 100.19±0.83 to 100.50±0.53. The results were compared favorably with the reported method.