Some new 2- phenyl benzimidazole derivatives were synthesised by cyclocondensation with appropriate reagents. The compounds synthesised were identified by 1H NMR, FAB Mass and FT-IR spectroscopic techniques. All compounds studied in this work were screened for their in vitro antimicrobial activities against the standard strains: Staphylococcus aureus ATCC - 25923, ATCC - 441 and Bacillus subtilis ATCC- 6633 as gram positive, Escherichia coli ATCC - 11775 and Pseudomonas aeruginosa ATCC 10145 as gram negative bacteria. Some of the compounds inhibited the growth of gram-positive bacteria (B. subtilis and S. aureus) at MIC values between 25 and 200 mg/mL. Some of the compounds exhibit antimicrobial activity against gram negative bacteria (E. coli and P. Aeruginosa) MIC values between 25 and 200 mg/mL.