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E-Journal of Chemistry
Volume 6, Issue 1, Pages 77-88

Investigation of Hopanoid Biomarkers in Lake Sediments by GC-MS and RP-HPLC-APCI-MS

Gamze Kavran Belin

University of Geneva, Chemistry Department, Laboratory of Mass Spectrometry, Boulevard d'Yvoy 16, CH-1221 Geneva-4, Switzerland

Received 14 July 2008; Accepted 10 August 2008

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Hopanoids are mainly derived from bacteriohopanpolyols that occur especially in bacteria, show the importance of bacterial lipid contributions in geological materials. In this work, GC-MS and RP-HPLC-APCI-MS analyses of hopanoid biomarkers in oxic and anoxic sediment samples from Lake Cadagno (Swiss Alps) and Lake Voua de la Motte (Haute Savoie, France) are presented and discussed. Samples were ultrasonically extracted, fractionated by flush chromatography on silica gel and derivatised before the analyses. Periodic acid/ sodium borohydride cleavage was used to identify highly functionalised hopanoids