Journal of Chemistry

Journal of Chemistry / 2009 / Article

Open Access

Volume 6 |Article ID 795896 | https://doi.org/10.1155/2009/795896

Prafulla Kumar Sahu, M. Mathrusri Annapurna, "Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography", Journal of Chemistry, vol. 6, Article ID 795896, 6 pages, 2009. https://doi.org/10.1155/2009/795896

Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography

Received08 Jan 2009
Accepted08 Mar 2009

Abstract

RP-HPLC analytical method for the estimation of nabumetone in pharmaceutical dosage forms was developed and validated. A Hypersil ODS C18, 4.6 mm x 250 mm, 5 μm column from Supelco (India), with mobile phase comprised of acetonitrile: triple distilled water (50:50) with a total run time of 18 min was used and the wavelength of the detector was set at 230 nm. Stavudin is used as internal standard. The retention times were 14.167 min and 1.967 min for nabumetone and stavudin (IS) respectively. The extraction recovery of nabumetone from pharmaceutical dosage form (tablets) was >101% and the calibration curve was linear (r2 = 0.995) over nabumetone concentrations ranging from 1 to 200 µg/mL. The method had an accuracy of >99% and LOD and LOQ of 0.17482 µg/mL and 0.5827 µg/mL respectively. The method reported is simple, reliable, precise and accurate and has the capability of being used for determination of nabumetone in bulk and pharmaceutical dosage forms.

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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