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E-Journal of Chemistry
Volume 7, Issue 1, Pages 117-122
http://dx.doi.org/10.1155/2010/490328

Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation

S. K. Patro,1 S. R. Swain,1 V. J. Patro,2 and N. S. K. Choudhury3

1Department of Pharmaceutical Analysis & Quality Assurance, Institute of Pharmacy and Technology, Salipur, Cuttack, Orissa-754202, India
2College of Pharmaceutical Sciences, Mohuda, Berhampur, Ganjam, Orissa, India
3Department of Pharmacy, S.C.B. Medical College, Cuttack, Orissa, India

Received 22 June 2009; Accepted 15 August 2009

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A new, simple, specific, accurate and precise RP-HPLC method was developed for determination of lamivudine in pure and tablet formulations. A Thermo BDS C18 column in isocratic mode, with a mobile phase consisting of 0.01 M ammonium dihydrogen orthophosphate buffer adjusted to pH 2.48 by using formic acid and methanol in the ratio of 50:50 was used. The flow rate was set at 0.6 mL/min and UV detection was carried out at 264 nm. The retention time of lamivudine and nevirapine were 2.825 min and 4.958 min respectively. The method was validated for linearity, precision, robustness and recovery. Linearity for lamivudine was found in the range of 50-175 μg/mL. Hence, it can be applied for routine quality control of lamivudine in bulk and pharmaceutical formulations.