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E-Journal of Chemistry
Volume 7, Issue 4, Pages 1206-1211
http://dx.doi.org/10.1155/2010/845862

Simultaneous HPTLC Estimation of Simvastatin and Ezetimibe in Tablet Dosage Form

Bhupendra Shrestha,1 B. Stephenrathinaraj,2 Sita Sharan Patel,3 N. K. Verma,4 and R. Mazumder5

1Department of Pharmaceutical Chemistry, Himalayan Pharmacy Institute, Majhitar, E.Sikkim-737136, India
2Department of Pharmaceutical Analysis, Vaagdevi College of Pharmacy, Hanamkonda, Warangal, Andhra Pradesh, India
3Department of Pharmacology, Himalayan Pharmacy Institute, Majhitar, E.Sikkim-737136, India
4Department of Pharmacology, Himalayan Pharmacy Institute, Majhitar, E.Sikkim-737136, India
5Department of Pharmaceutics, Calcutta Institute of Pharmaceutical Technology and AHS, Howrah, West Bengal-711316, India

Received 27 November 2009; Accepted 20 January 2010

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A method enabling the precise and quick simultaneous analysis of simvastatin and ezetimibe in tablet formulation by high performance thin layer chromatography has been presented. Samples of simvastatin and ezetimibe in organic solvents were separated on a plate coated with silica gel 60 F-254 and the chromatograms were developed using a mixture of chloroform and methanol (9.5: 0.5 %v/v). The method has a linearity range of 40-280 ng.mL-1 for both the drugs when scanned at 254 nm. The limit of detection and limit of quantitation was found to be 30 and 100 ng.band-1 respectively, for both the drugs. The mean percent recovery was found to be 100.65 and 101.55 for simvastatin and ezetimibe. The intra-day and inter-day precision studies were carried out with mean RSD of 0.88 and 1.27 for ezetimibe and 1.35 and 1.50 for simvastatin.