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E-Journal of Chemistry
Volume 8, Issue 2, Pages 483-490
http://dx.doi.org/10.1155/2011/234640

Stability-Indicating RP-HPLC Method for Assay of Silver Lactate

V. Srinivasan,2 H. Sivaramakrishnan,1 and B. Karthikeyan2

1Department of Analytical DevelopmentPiramal Healthcare Ltd, Ennore, Chennai-600 057, India
2Department of Chemistry Annamalai University, Annamalainagar-608002, India

Received 27 March 2010; Revised 31 May 2010; Accepted 17 July 2010

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple, economic and time-efficient stability-indicating, reverse-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for analysis of silver lactate in the presence of degradation products generated by decomposition. When silver lactate was subjected to acid hydrolysis, base hydrolysis, oxidative, photolytic, humidity and thermal stress, degradation was observed during base hydrolysis, oxidation, humidity and thermal stress. The drug was found to be stable to other stress conditions. Successful chromatographic condition of the drug from the degradation products formed under stress conditions was achieved on a phenomenex Gemini column with potassium dihydrogen phosphate buffer, pH adjusted to 2.2 with orthophosphoric acid, as mobile phase. The method was validated for linearity, precision, specificity and robustness and can be used for quality-control during manufacture and assessment of the stability of samples of silver lactate. To the best of our knowledge, a validated stability-indicating LC assay method for silver lactate based on lactic acid is reported for the first time.