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E-Journal of Chemistry
Volume 8, Issue 3, Pages 1430-1437
http://dx.doi.org/10.1155/2011/428019

Antimicrobial Activity and HPLC Fingerprinting of Crude Ocimum Extracts

S. S. Deo, F. Inam, and R. P. Mahashabde

Chemistry Department, Institute of ScienceRabindranath Tagore Road, Civil Lines, Nagpur-440010, India

Received 19 August 2010; Accepted 8 November 2010

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The antimicrobial activity of crude methanolic and aqueous extracts of Ocimum sanctum and Ocimum kilimandsacharicum against gram positive, gram negative and antifungal activity was evaluated to find the zone of inhibition and to set a HPLC profile or fingerprint of these extracts. The crude methanolic extract of Ocimum sanctum showed strong antimicrobial activity against S.aureus and C. albicans and moderate activity against E. coli and B. subtilis. The crude methanolic extract of Ocimum kilimandsacharicum showed strong antimicrobial activity against S. aureus, E. coli and C. albicans at higher concentration, same as that shown by the standard for C. albicans. It showed moderate activity against B. subtilis. The crude aqueous extracts of Ocimum sanctum showed strong antimicrobial activity against S.aureus and moderate against others. Whereas the crude aqueous extracts of Ocimum kilimandsacharicum showed moderate activity against the gram positive and gram negative organisms and strong activity against C. albicans at higher concentration, same as that shown by the standard for C. albicans.