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E-Journal of Chemistry
Volume 8 (2011), S1, Pages S41-S46

A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations

Prafulla Kumar Sahu,1 M. Mathrusri Annapurna,2 and Dillipkumar Sahoo2

1Department of Pharmaceutical Analysis & Quality Assurance, Nimra College of Pharmacy, Nimra Nagar, Ibrahimpatnam, Vijayawada, Andhra Pradesh, 521456, India
2Department of Pharmaceutical Analysis Roland Institute of Pharmaceutical Sciences Khodasing, Berhampur, Ganjam, Orissa, 760010, India

Received 27 January 2011; Accepted 12 April 2011

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper describes a high-performance liquid chromatographic method for simultaneous estimation of nabumetone and paracetamol in binary mixture. The method was based on RP-HPLC separation and quantitation of the two drugs on hypersil C-18 column (250 mm × 4.6 mm) using a mobile phase consisting of acetonitrile and 0.05% aqueous acetic acid (70:30v/v) at flow rate of 1 mL min-1. Quantitation was achieved with PDA detector at 238 nm based on peak area with linear calibration curves at concentration ranges 5-25 µg mL-1 for both the drugs. Naproxen sodium was used as internal standard. The method has been successively applied to pharmaceutical formulation. No chromatographic interference from the tablet excipients was found. The method was validated in terms of precision, robustness, recovery and limits of detection and quantitation. The intra and inter-day precision and accuracy values were in the acceptance range as per ICH guidelines.