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E-Journal of Chemistry
Volume 8 (2011), Issue 4, Pages 1620-1625

Simultaneous HPLC Determination of Methocarbamol, Paracetamol and Diclofenac Sodium

Deshmukh Hafsa, S. Chanda, and Pradnya J. Prabhu

Department of Chemistry, K. J. Somaiya College of Science and Commerce, Vidyavihar (E), Mumbai-400077, India

Received 18 December 2010; Accepted 28 February 2011

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A rapid and simple high performance liquid chromatography method was developed and validated at λ=275 nm for simultaneous determination of methocarbamol, paracetamol and diclofenac sodium (lamotrigine as an internal standard) from pharmaceutical preparation. The separation was performed using methanol: water: GAA, in the ratio of 400:600:05 (v/v) as the mobile phase. The detector response was linear in the range of 5 to 45 μg, 3.25 to 29.25 μg, 0.5 to 4.5 μg for methocarbamol, paracetamol and diclofenac sodium respectively. The percentage assay of methocarbamol, paracetamol and diclofenac sodium was found between 100, 99.76 and 99.31% respectively. The described method has the advantage of being rapid and easy hence it can be applied for routine quality control analysis.