Table of Contents Author Guidelines Submit a Manuscript
E-Journal of Chemistry
Volume 8, Issue 2, Pages 587-593

Third Derivative Spectrophotometric Method for Simultaneous Determination of Copper and Nickel Using 6-(2-Naphthyl)-2, 3-dihydro-1,2,4- triazine-3-thione

Maliheh Barazandeh Tehrani and Effat Souri

Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran

Received 6 July 2010; Accepted 3 September 2010

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple and sensitive derivative spectrophotometric method for simultaneous determination of nickel and copper using 6-(2- naphthyl)-2,3-dihydro-1,2,4-triazine-3-thione (NDTT) as a selective analytical reagent was developed. The complexes of metal ions with NDTT were formed immediately in basic media and extracted with chloroform. The zero-crossing measurement technique was found suitable for the direct measurement of the third-derivative value of Ni-NDTT and Cu-NDTT at 501 and 472 nm respectively. Beer’s law is obeyed in the concentration range 1-30 μg/mL for both cations with different ratios. The limit of detection was 0.26 μg/mL and 0.13 μg/mL for copper and nickel respectively. The within day and between day variations showed coefficient of variation (CV%) values less than 2.80 and 2.97 for copper and nickel respectively. The error of the determination method did not exceed 6.50%. Analysis of alloy sample showed that this method can be successfully used for simultaneous determination of Cu and Ni in real samples.