Synthesis and In Vitro Cytotoxic Activity of 2-Amino-7-(dimethylamino)-4-[(trifluoromethyl)phenyl]-4H-chromenes

Abstract

Three 2-amino-4-(trifluoromethylphenyl)-3-cyano-7-(dimethylamino) -4H-chromene derivatives were synthesized and their cytotoxic activities were determined against six human tumor cell lines using MTT assay. Condensation of 3-(dimethylamino)phenol, trifluoromethybenzaldehydes and malonitrile in ethanol containing piperidine afforded corresponding chromenes (4a-c). The structure of the synthesized compound was confirmed by ¹H NMR, IR and Mass spectral data. Among compounds tested, 3-trifluoromethyl analogue (3b) was the most active against all human tumor cell lines (IC₅₀=12-45 nM).