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E-Journal of Chemistry
Volume 9, Issue 1, Pages 340-344

Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method

V. Bhaskara Raju2 and A. Lakshmana Rao1

1V.V. Institute of Pharmaceutical Sciences, Gudlavalleru-521 356, A.P., India
2Sri Vasavi Institute of Pharmaceutical Sciences, Tadepalligudem- 534 101, A.P., India

Received 25 June 2011; Accepted 13 August 2011

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An accurate and precise HPLC method was developed for the determination of lisinopril. Separation of the drug was achieved on a reverse phase C8 column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 35:65v/v. The flow rate was 0.8 mL/min and the detection wavelength was 215 nm. The linearity was observed in the range of 20-60 μ g/mL with a correlation coefficient of 0.9992. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of lisinopril in tablet dosage forms.