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E-Journal of Chemistry
Volume 9, Issue 2, Pages 700-704
http://dx.doi.org/10.1155/2012/359745

New Spectrophotometric Method for the Determination of Bosentan - An Anti-Hypertensive Agent in Pharmaceutical Dosage Forms

1Department of Pharmaceutical analysis and Quality Assurance, Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa, India
2Royal College of Pharmacy and Health Sciences, Berhampur, Orissa, India
3GITAM Institute of Pharmacy, GITAM University, Visakhapatnam, India

Received 23 July 2011; Accepted 20 September 2011

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A new, simple and sensitive spectrophotometric method in ultraviolet region has been developed for the determination of Bosentan in bulk and in pharmaceutical formulations. Bosentan exhibits absorption maxima at 273 nm with apparent molar absorptivity of 1.3293×104 L/mol.cm in methanol and octane 1-sulfonic acid solvent mixture. Beer's law was found to be obeyed in the concentration range of 0.1-100 μg/mL. The method was validated as per the ICH guidelines.