A rapid, sensitive and precise HPLC method was developed for the estimation of trospium chloride in pure and tablet dosage forms. Seperation of the drug was achieved on a reverse phase Azilent C18 column using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 60:40v/v. The flow rate was 1 ml/min and the detection wave length 215 nm. The linearity was found in the range of 10-150 μg/ml with a correlation coefficient of 1.0000. The proposed method was validated for its sensitivity, linearity, accuracy and precision. This method was employed for routine quality control analysis of trospium chloride in tablet dosage forms.