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E-Journal of Chemistry
Volume 9 (2012), Issue 1, Pages 224-232

Simultaneous Determination of Eosin-Yellow and Ponceau-S Using H-Point Standard Addition Method in Micellar Media

Amandeep Kaur and Usha Gupta

Department of Chemistry, Punjabi University, Patiala-147002, Punjab, India

Received 9 June 2011; Accepted 12 August 2011

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


H-point standard addition method (HPSAM) is developed for simultaneous determination of eosin-Y and ponceau-s in micellar media. Nickel chloride (NiCl2) is used as chromogenic reagent for complexes formation of eosin-Y and ponceau-S food colorants. The measurements were carried out using sodium lauryl sulphate as a surfactant, in buffered solution at pH 6.0. The concentration range of 0.115-2.53 μg/mL of eosin-Y and 0.159-3.80 μg/mL of ponceau-S. The proposed procedures have been applied successfully for the simultaneous determination of eosin-Y and ponceau-S in synthetic binary mixtures and real samples.