An Overview of Analytical Determination of Diltiazem, Cimetidine, Ranitidine, and Famotidine by UV Spectrophotometry and HPLC Technique

Table 1

Official methods of analysis.

Drug	Column	Mobile phase	nm	Ref.
Diltiazem	Column L1 4.6 × 300 mm	Buffer : MeOH : ACN (50 : 25 : 25).	240	[15]; USP
Cimetidine	Column L1 4.6 × 250 mm	MeOH : H3PO4 : (Sod, Hex sulphonate) (240 mL : 0.3 mL : 940 mg) make up to 1 L	220	USP; [16]
	Column C18 4.6 × 250 mm	Buffer (1.1 g Sod, Hex sulphonate in 780 mL water + 0.4 mL diethyl amine, pH 2.8 by H3PO4) + 250 mL MeOH	220	B.P; [17]
Ranitidine	Column L1 4.6 × 200 mm	MeOH : buffer (85 : 15),; buffer: 0.1 M ammonium cyanate	322	USP; [18]
Famotidine	Column L1 4.6 × 250 mm	Water : MeOH : buffer (32 : 5 : 3), buffer: 13.8 g NaH2PO4 in water—1 L	254	USP; [19]
	Column C18 4.6 × 250 mm, temp: 50°C	MeOH : ACN : buffer (6 : 94 : 900), buffer (1.88 g Sod, Hex sulphonate) in 900 mL water—ph 3.5 by acetic acid	265	B.P; [20]