RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals

Table 2

Results of validation parameters.

Parameter	CEFE	TAZO
System suitability parameters (𝑛=5)
Retention time (min) ± % RSD	10.49 ± 0.68	7.69 ± 0.83
Capacity factor ± % RSD	3.19 ± 0.35	2.07 ± 0.84
Theoretical plate ± % RSD	10505 ± 1.11	12957 ± 0.65
Asymmetry factor ± % RSD	1.02 ± 0.92	0.99 ± 1.24
Linearity study
Linearity range (μg/mL)	4–24	0.5–3.0
Slope	73730	48802
Intercept	27477	2327.9
Correlation coefficient (𝑟^2)	0.9977	0.9974
Precision (𝑛=3)
Repeatability (% RSD)	0.92	1.09
Interday (% RSD)	0.27	0.54
Intraday (% RSD)	0.51	0.29
Accuracy (𝑛=3)
80% ± (% RSD)	102.22 ± 0.25	99.92 ± 1.09
100% ± (% RSD)	103.01 ± 0.93	102.02 ± 0.76
120% ± (% RSD)	100.95 ± 0.68	99.82 ± 1.34
Specificity study	Percent drug remained
Acid stress	95.94	82.29
Base stress	94.95	98.63
Oxidation stress	67.19	87.4
Thermal stress	92.28	91.27