Aqueous Two-Phase Systems: A New Approach for the Determination of Brilliant Blue FCF in Water and Food Samples

Figure 2

Effect of volume of buffer on the extraction efficiency. Experimental conditions: pH of sample; 5.5, amount of PEG 4000; 4 gm, amount of sodium carbonate; 2.5 gm, temperature; 35°C, Centrifuging time; 10 min, 4 ng/mL of brilliant blue.