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Journal of Chemistry
Volume 2013 (2013), Article ID 325372, 5 pages
http://dx.doi.org/10.1155/2013/325372
Research Article

Evaluation of Taurine by HPTLC Reveals the Mask of Adulterated Edible Bird’s Nest

Key Laboratory of Animal Resistance, College of Life Science, Shandong Normal University, 88 East Wenhua Road, Jinan 250014, China

Received 27 May 2013; Accepted 17 July 2013

Academic Editor: Lian-Wen Qi

Copyright © 2013 Peishan Teo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Detection of amino acid is an effective and common method to determine adulteration in edible bird’s nest. Therefore, a simple and sensitive method was developed to detect taurine for determining adulteration in edible bird’s nest in the future. Sample was separated on precoated silica gel GF254 high-performance thin layer chromatographic plates. Separation of taurine was performed by n-propyl alcohol : ethanol : water : glacial acetic acid (5.2 : 0.8 : 2 : 2, v : v : v : v). Densitometric analysis of taurine was carried out in the absorbance mode at 485 nm. The method was validated for precision, intra- and interday variation, and recovery. This study proved that high-performance thin layer chromatography is a simple, rapid, precise, and selective method for qualitative and quantitative analysis of taurine in edible bird’s nest.