Analysis of A-Type and B-Type Highly Polymeric Proanthocyanidins and Their Biological Activities as Nutraceuticals

Table 3

Proportions of extension units in highly polymeric proanthocyanidins from seed shells of Japanese horse chestnut, blueberry, and cranberry as determined by UPLC-ESI/MS of the dodecylsulfide derivatives after thiolytic cleavage.

	Cleaved extension unit	Proportion (mol%)
		AF2	AF3	BF2	BF3	CF2	CF3
4	(Epi)gallocatechin dodecylsulfide	1.9	1.7	1.5	3.1	7.6	6.3
5	(Epi)gallocatechin-(epi)catechin A-type dimer dodecylsulfide	3.6	2.8	N.D.	N.D.	N.D.	N.D.
6	(Epi)catechin A-type trimer dodecylsulfide	0.9	0.8	N.D.	N.D.	N.D.	N.D.
7	(Epi)catechin A-type dimer dodecylsulfide	32.2	25.3	6.8	6.4	25.4	20.3
8	(Epi)catechin dodecylsulfide	61.3	69.4	91.6	90.6	67.0	73.3

The dodecylsulfide derivatives of extension units were quantified by monitoring the absorbance at 280 nm. Data represent the mean of three independent experiments. N.D.: not detectable.