Determination of Retinol, 𝛼-Tocopherol, Lycopene, and 𝛽-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study

Figure 2

An HPLC chromatogram of retinol (1.14 μmol/L; 0.33 μg/mL),  𝛼-tocopherol (27.3 μmol/L; 11.76 μg/mL), lycopene (0.021 μmol/L; 0.011 μg/mL), and β-carotene (0.073 μmol/L; 0.039 μg/mL) in human plasma. Peaks: (1) retinol, (2) retinyl acetate, (3)  𝛼-tocopherol, (4)  𝛼-tocopheryl acetate, (5) lycopene, and (6) β-carotene. HPLC conditions: see Figure 1.