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Journal of Chemistry
Volume 2013, Article ID 484389, 5 pages
http://dx.doi.org/10.1155/2013/484389
Research Article

Extractive Spectrophotometric Method for the Determination of Lamivudine and Zidovudine in Pharmaceutical Preparations Using Bromocresol Purple

Bezmialem Vakif University, Department of Analytical Chemistry, Faculty of Pharmacy, Fatih, 34093 Istanbul, Turkey

Received 1 February 2013; Accepted 18 July 2013

Academic Editor: Pranav S. Shrivastav

Copyright © 2013 Serife Evrim Kepekci Tekkeli. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A new spectrophotometric method has been established for the quantitation of lamivudine (LVD) and zidovudine (ZVD) in pharmaceutical preparations. The method is based on the reaction between the investigated drug substances and bromocresol purple (BCP) producing ion-pair complexes in acidic buffers which are suitable for chloroform extraction. The maximum absorbance of these complexes was measured at 424 nm in chloroform. All variables were studied to optimize the reaction conditions. Linearity ranges were found to be 25–250 μg mL−1 for LVD-BCP and 50–300 μg mL−1 for ZVD-BCP. The developed method was successfully applied for the determination of these drugs in pharmaceutical preparations. Excipients in pharmaceutical formulations did not interfere in the analysis. The results were compared statistically with those obtained by the HPLC method reported in the literature. According to the results, the proposed method can be recommended for quality control and routine analysis.