Optimization of Synthesis of Seleno-Sargassum fusiforme (Harv.) Setch. Polysaccharide by Response Surface Methodology, Its Characterization, and Antioxidant Activity

Table 2

Box-Behnken design of three variables and the experimental results.

No.	𝑋1/reaction time (h)	𝑋2/reaction temperature (°C)	𝑋3/ratio of Na2SeO3 to SFPSI (g/g)	Se content in Se-SFPSI (mg/g)
1	−1 (7)	−1 (60)	0 (0.8)	2.117
2	1 (9)	−1 (60)	0 (0.8)	2.675
3	−1 (7)	1 (80)	0 (0.8)	2.777
4	1 (9)	1 (80)	0 (0.8)	2.726
5	−1 (7)	0 (70)	−1 (0.6)	2.675
6	1 (9)	0 (70)	−1 (0.6)	3.005
7	−1 (7)	0 (70)	1 (1.0)	2.904
8	1 (9)	0 (70)	1 (1.0)	3.030
9	0 (8)	−1 (60)	−1 (0.6)	2.371
10	0 (8)	1 (80)	−1 (0.6)	2.980
11	0 (8)	−1 (60)	1 (1.0)	2.827
12	0 (8)	1 (80)	1 (1.0)	3.056
13	0 (8)	0 (70)	0 (0.8)	3.234
14	0 (8)	0 (70)	0 (0.8)	3.208
15	0 (8)	0 (70)	0 (0.8)	3.259
16	0 (8)	0 (70)	0 (0.8)	3.234
17	0 (8)	0 (70)	0 (0.8)	3.183