Research Article
Production, Purification, and Characterization of Thermostable α-Amylase Produced by Bacillus licheniformis Isolate AI20
Table 4
Purification scheme of α-amylase enzyme extracted from Bacillus licheniformis AI20.
| | Purification step | Volume (mL) | α-amylase activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
| | Culture filtrate | 148 | 4389.68 | 347.80 | 12.621 | 100 | 1 | | Concentrated culture | 45 | 3055.95 | 196.65 | 15.540 | 69.617 | 1.231 | | DEAE Sepharose FF | 27 | 2014.20 | 18.90 | 106.572 | 45.885 | 8.444 | | Sephacryl S-100 HR | 5 | 554.25 | 0.74 | 748.986 | 12.626 | 59.344 |
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