Validated HPTLC Method for Simultaneous Analysis of Pyrimethamine and Sulphadoxine in Pharmaceutical Dosage Forms

Table 1

Results from method validation.

Property	Value
	PYR	SUL
Range (ng per spot)	100–500	2000–10000
Correlation coefficient (𝑟^2)	0.9998	0.9987
Slope	12.128	3.421
Limit of detection (ng per band)	14.15	58.37
Limit of quantification (ng per band)	43.07	152.26
Specificity	Specific	Specific
System suitability, RSD (%)	0.93	1.20

PYR: pyrimethamine; SUL: sulphadoxine.