Benzophenone Suppression of Quercetin Antioxidant Activity towards Lipids under UV-B Irradiation Regime: Detection by HPLC Chromatography
Degradation (bleaching) of quercetin from the UV-B-irradiated sample, that is, quercetin/BZP/phospholipids mixture in methanol. (a) The recorded samples chromatograms recorded at 371 nm (quercetin retention time, , of 2.6 min). (b) Changes in quercetin absorption spectra taken from the upper (a) chromatogram (quercetin peak at min), as a result of the increasing UV-B irradiation periods . (c) Kinetics of UV-B irradiation-induced bleaching, followed through a decrease of quercetin peak ( min) integrated areas as a result of the increased UV-B irradiation periods, . The corresponding bleaching rate constant and the related error bars are shown in the plot.