Antibacterial, Cytotoxic Studies and Characterization of Some Newly Synthesized Symmetrical N³,N^3′-Bis(disubstituted)isophthalyl-bis(thioureas) and Their Cu(II) and Ni(II) Complexes

Table 2

In vitro antibacterial activity of ligands (L¹–L⁵) and their metal(II) complexes.

Compounds	Test microorganisms/diameter of inhibition zone (IZ in mm)
	Gram-positive bacteria		Gram-negative bacteria
	B. subtilis	S. aureus	E. coli	S. sonnei	S. typhi	P. aeruginosa
L^1	34 ± 0.83^b	33 ± 1.67^c	35 ± 1.35^b	33 ± 1.64^b	31 ± 1.12^b	25 ± 0.76^c
L^2	28 ± 0.81^c	32 ± 2.11b^c	31 ± 0.75^c	32 ± 0.63^d	27 ± 1.63^b	28 ± 0.81^b
L^3	32 ± 1.67^b	29 ± 1.45^c	31 ± 1.23^b	30 ± 1.63^b	29 ± 0.0^b	22 ± 0.70^c
L^4	34 ± 0.83^b	33 ± 1.42^c	32 ± 1.63^b	32 ± 0.63^d	27 ± 1.63^b	35 ± 1.35^b
L^5	—	—	—	—	—	—
C^1	37 ± 1.48^b	36 ± 1.34^c	37 ± 0.78^b	35 ± 1.34^b	34 ± 1.7^b	29 ± 0.74^c
C^2	29 ± 0.86^c	30 ± 2.11^bc	24 ± 0.75^c	21 ± 0.68^d	30 ± 1.65^b	31 ± 0.84^b
C^3	33 ± 1.67^b	29 ± 1.45^c	32 ± 1.63^b	34 ± 1.63^b	29 ± 0.0^b	22 ± 0.70^c
C^4	35 ± 0.81^e	36 ± 1.45^c	32 ± 1.63^b	33 ± 0.66^d	35 ± 1.20^a	32 ± 0.70^c
C^5	34 ± 0.41^c	35 ± 1.25^b	31 ±1.20^a	34 ± 0.45^a	36 ± 1.0^f	30 ± 0.50^b
C^6	35 ± 0.83^b	34 ± 1.67^c	36 ± 1.35^b	37 ± 1.64^b	31 ± 1.12^b	31 ± 0.76^c
C^7	30 ± 0.85^c	31 ± 2.37^bc	29 ± 0.87^c	27 ± 0.66^d	31 ± 1.69^b	32 ± 0.83^b
C^8	34 ± 0.45^f	28 ± 0.21^f	33 ± 0.91^b	31 ± 1.30^e	30 ± 1.21^c	26 ± 0.81^e
C^9	34 ± 2.11^bc	35 ± 0.85^c	33 ± 0.0^b	34 ± 1.45^c	30 ± 1.44^c	31 ± 1.69^b
C^10	33 ± 0.01^b	34 ± 0.25^c	31 ± 0.10^c	33 ± 1.05^a	30 ± 1.04^c	31 ± 1.09^b
S^1	38 ± 0.41^a	36 ± 1.82^a	38 ± 1.61^a	36 ± 1.63^a	35 ± 1.63^a	34 ± 1.47^a
S^2	38 ± 0.81^a	35 ± 1.41^a	38 ± 1.20^a	37 ± 0.0^a	34 ± 0.0^a	33 ± 0.0^a

S¹: Ampicillin, S²: Ciprofloxacin, —: NIL.
Values are mean ± SD of three separate experiments.
Letters in superscript show the significance of the results against single strain.